Canadian Forest Service Publications

Determination of abamectin in some forest matrices by liquid chromatography with flueorescence detection. 1997. Sundaram, K.M.S.; Curry, J. Journal of Liquid Chromatography and Related Technology. 20:1757-1772.

Year: 1997

Issued by: Great Lakes Forestry Centre

Catalog ID: 34060

Language: English

CFS Availability: PDF (request by e-mail)

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Abstract

A liquid chromatographic method was developed and validated for the determination of abamectin Bu residues in some terrestrial(balsam firandoak Mage, sandy andclay loam soils and leaflitter)and aquatic (stream water and sediment) forest matrices. The processed foliage, soil, litter, and sediment were fortified with abamectin andextracted with ethyl acetate. The fortified steam water was extracted with dichloromethane. Aliquots of crude extracts were cleaned with Florisil® column chromatography and the purified extracts were derivatized using 1-methylimidazole and trifluoroacetic anhydride. The derivatized abamectin was analysed by reverse phase liquid chromatography, with a fluorescence detector set at 232 nm excitation and 461 nm emission wavelengths. ASpherisorb® ODS2, 5(om, 250 x4 mm column was used. The samples were run isocratically using methanol-water as the mobile phase. Mean recoveries for the analyte ranged from 83.0 to 93.0%, with a coefficient of variation from 6.3 to 12.4%. Limitsof detection and limits of quantitation for solid matrices ranged from 0.10 to 0.20 and from 0.30 to 0.60 ng/g, respectively, and for stream water the corresponding values were 0.003 and 0.009 ng/mL. The procedure provides a reliable and sensitive method for determining abamectin Bia residues in forest matrices.