Canadian Forest Service Publications

Monoclonal antibodies and their application in forestry. 1991. Ekramoddoullah, A.K.M. Pages 161-168 in J.R. Sutherland and S.G. Glover, Editors. Proceedings of the first meeting of IUFRO Working Party S2.07-09 (Diseases and Insects in Forest Nurseries), August 23-30, 1990, Victoria, BC. Forestry Canada, Pacific Forestry Centre, Victoria, BC, Information Report BC-X-331. 298 p.

Year: 1991

Issued by: Pacific Forestry Centre

Catalog ID: 3138

Language: English

CFS Availability: Order paper copy (free)

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In this paper, a few basic concepts of immunology are introduced. These concepts are (i) antibody structure, (ii) functional fragments of antibody molecules, (iii) antigen-antibody interactions, (iv) antibody specificity and antibody affinity, (v) antigen cross-reactivity, (vi) cells involved in the antibody response, (vii) clonal selection, (viii) hybridoma technology, (ix) immunological techniques to screen hybridoma supernatant for antibody activity. The application of monoclonal antibodies was discussed in terms of their use in (a) affinity chromatography to isolate a specific antigen, (b) establishing structure-function relationships, (c) detection of seed-borne pathogens and (d) the study of host-pathogen interactions. Possible applications of these techniques in forestry are discussed.