Canadian Forest Service Publications

An intracellular symbiont and other microbiota associated with field-collected populatios of sawflies (Hymenoptera: Symphyta) 2008. Graham, R.I.; Zahner, V.; Lucarotti, C.J. Canadian Journal of Microbiology 54: 758-768.

Year: 2008

Issued by: Atlantic Forestry Centre

Catalog ID: 28912

Language: English

CFS Availability: PDF (download)

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Abstract

Six species of sawfly (Hymenoptera: Symphyta) from four taxonomic families (Agridae, Diprionidae, Pamphiliidae, and Tenthredinidae) were collected from locations across Canada and surveyed four their associated microbiota. Total DNA was extracted from individual insects, and polymerase chain reaction (PCR) was used to amplify the conserved 16S rRNA gene from microbiota. Denaturing gradient gel electrophoresis (DGGE) and restriction fragment length polymorphism (RFLP) were undertaken to separate bacterial clones associated with the host insect. Sequencing of the PCR-DGGE and PCR-RFLP products revealed a dominance of alpha- and gamma-Proteobacteria, with most sequences showing high similiarity to bacteria previously identified from other insect species and environmental samples. Additionally, a strain of the bacterial endosymbiont Wolbachia and a Wolbachia bacteriophage were identified from the mountain ash sawfly (Pristiphora geniculata).